Journal: International journal of oral science

Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.

doi: 10.1038/s41368-022-00192-2

Figure Lengend Snippet: Fig. 2 OSCC-derived sEVs enhance the proliferation and inhibit the apoptosis of normal epithelial cells. a, b The proliferative cells of HIOECs treated with OSCC-derived sEVs. Scale bar, 50 μm. c The size and morphology of HIOECs in Matrigel treated with OSCC-derived sEVs. Scale bar, 50 μm. d Immunofluorescence staining of Ki67 in HIOECs treated with OSCC-derived sEVs in Matrigel. Scale bars, 100 μm. e, f The apoptosis rate of HIOECs treated with OSCC-derived sEVs. g The expression of apoptosis-related markers in HIOECs treated with OSCC-derived sEVs

Article Snippet: EdU assay The assay was carried out as previously described.43 In summary, HIOECs were uniformly plated into 24-well plates, fixed and stained using the EdU Cell Proliferation Kit (Beyotime, China) as instructed, and finally observed and photographed under a fluorescence microscope (Keyence, Japan).

Techniques: Derivative Assay, Staining, Expressing

Journal: International journal of oral science

Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.

doi: 10.1038/s41368-022-00192-2

Figure Lengend Snippet: Fig. 5 MiRNA let-7c plays a similar role to OSCC-derived sEVs on normal epithelial cells. a The proliferation ability of HIOECs transfected with let-7c mimics. b The proliferative cells of HIOECs transfected with let-7c mimics. c The size and morphology of HIOECs transfected with let-7c mimics in Matrigel. Scale bar, 50 μm. d Immunofluorescence staining of Ki67 in HIOECs transfected with let-7c mimics in Matrigel. Scale bars, 100 μm. e, f The apoptosis rate of HIOECs transfected with let-7c mimics. g The expression of apoptosis-related markers in HIOECs transfected with let-7c mimics. h The expression of EMT-related markers in HIOECs transfected with let-7c mimics

Article Snippet: EdU assay The assay was carried out as previously described.43 In summary, HIOECs were uniformly plated into 24-well plates, fixed and stained using the EdU Cell Proliferation Kit (Beyotime, China) as instructed, and finally observed and photographed under a fluorescence microscope (Keyence, Japan).

Techniques: Derivative Assay, Transfection, Staining, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Ginsenoside Rh2 Ameliorates Doxorubicin-Induced Senescence Bystander Effect in Breast Carcinoma Cell MDA-MB-231 and Normal Epithelial Cell MCF-10A

doi: 10.3390/ijms20051244

Figure Lengend Snippet: Rh2 suppressed potential signaling pathways regulating SASP in breast cell lines. Senescent MDA-MB-231 ( A ) and MCF-10A ( B ) cells treated with or without Rh2 were lysed and subjected to the Signaling Nodes Multitarget Sandwich ELISA assay. ** indicates p < 0.01 versus nontreated con; *** indicates p < 0.001 versus nontreated con. ## indicates p < 0.01 versus CM alone group. ### indicates p < 0.001 versus CM alone group.

Article Snippet: To address the potential signaling pathways that activated SASP, we investigated the major pathways that regulate cell growth, differentiation, response to proinflammatory cytokines, growth factor receptors, inflammation, and cellular stress in the Signaling Nodes Multitarget Sandwich ELISA Kit.

Techniques: Protein-Protein interactions, Sandwich ELISA